ARG65572
anti-NDUFAF4 antibody
anti-NDUFAF4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFAF4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFAF4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of human NDUFAF4 |
| Conjugation | Un-conjugated |
| Alternate Names | HSPC125; C6orf66; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4; Hormone-regulated proliferation-associated protein of 20 kDa; HRPAP20; bA22L21.1; My013 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Raji cell |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 2.8 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9P032 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 |
|---|---|
| Gene Symbol | NDUFAF4 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) complex I, assembly factor 4 |
| Background | NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency. |
| Function | Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 20 kDa |
| PTM | Phosphorylated on serine. Prolactin stimulate serine phosphorylation (By similarity). |
Images (3) Click the Picture to Zoom In
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ARG65572 anti-NDUFAF4 antibody WB image
Western blot: 40 μg of Raji cell lysate stained with ARG65572 anti-NDUFAF4 antibody at 1:300 dilution. Exporsure time: 5 seconds.
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ARG65572 anti-NDUFAF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65572 anti-NDUFAF4 antibody (left) at 1:45 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
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ARG65572 anti-NDUFAF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65572 anti-NDUFAF4 antibody (left) at 1:45 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
