ARG43967

anti-NDUFAF4 antibody

anti-NDUFAF4 antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFAF4
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFAF4
Antigen Species Human
Immunogen Human NDUFAF4 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
Alternate Names NDUFAF4; NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 4; HRPAP20; BA22L21.1; HSPC125; C6orf66; My013; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 4 ; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 4; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 4; Hormone-Regulated Proliferation-Associated Protein Of 20 KDa; Hormone-Regulated Proliferation-Associated Protein, 20 KDa; Chromosome 6 Open Reading Frame 66; MC1DN15

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg /1x10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 29078 Human NDUFAF4

Swiss-port # Q9P032 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4

Gene Symbol NDUFAF4
Gene Full Name NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 4
Background NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.
Function Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I)
Cellular Localization Membrane, Mitochondrion
Calculated MW 20 kDa
PTM Lipoprotein, Myristate, Phosphoprotein

Images (6) Click the Picture to Zoom In

  • ARG43967 anti-NDUFAF4 antibody IHC-P image

    Immunohistochemistry: Human lung adenocarcinomastained with ARG43967 anti-NDUFAF4 antibody at 2 μg/ml dilution.

  • ARG43967 anti-NDUFAF4 antibody WB image

    Western blot: 293T stained with ARG43967 anti-NDUFAF4 antibody at 0.5 μg/mL dilution.

  • ARG43967 anti-NDUFAF4 antibody IHC-P image

    Immunohistochemistry: Human liver cancer with ARG43967 anti-NDUFAF4 antibody at 2 μg/ml dilution.

  • ARG43967 anti-NDUFAF4 antibody WB image

    Western blot: K562 stained with ARG43967 anti-NDUFAF4 antibody at 0.5 μg/mL dilution.

  • ARG43967 anti-NDUFAF4 antibody FACS image

    Flow Cytometry: U937 cells stained with ARG43967 anti-NDUFAF4 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43967 anti-NDUFAF4 antibody IHC-P image

    Immunohistochemistry: Human ovarian tumor with ARG43967 anti-NDUFAF4 antibody at 2 μg/ml dilution.