ARG65570
anti-NDUFAF1 antibody
anti-NDUFAF1 antibody for Western blot and Human
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFAF1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFAF1 |
| Antigen Species | Human |
| Immunogen | Fusion protein of human NDUFAF1 |
| Conjugation | Un-conjugated |
| Alternate Names | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1; Complex I intermediate-associated protein 30, mitochondrial; CGI-65; CGI65; CIA30 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | 231 and Jurkat cell |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y375 Human Complex I intermediate-associated protein 30, mitochondrial |
|---|---|
| Gene Symbol | NDUFAF1 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) complex I, assembly factor 1 |
| Background | This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. |
| Function | Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 38 kDa |
Images (1) Click the Picture to Zoom In
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ARG65570 anti-NDUFAF1 antibody WB image
Western blot: 40 μg of 1) 231 and 2) Jurkat cell lysates stained with ARG65570 anti-NDUFAF1 antibody at 1:500 dilution. Exposure time: 20 seconds.
