ARG65569
anti-NDUFA9 antibody
anti-NDUFA9 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFA9 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFA9 |
| Antigen Species | Human |
| Immunogen | Fusion protein of human NDUFA9 |
| Conjugation | Un-conjugated |
| Alternate Names | CI-39k; SDR22E1; Complex I-39kD; CC6; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial; CI39k; CI-39kD; NADH-ubiquinone oxidoreductase 39 kDa subunit; NDUFS2L |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse heart and kidney tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 1.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16795 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondria Swiss-port # Q9DC69 Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondria |
|---|---|
| Gene Symbol | NDUFA9 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa |
| Background | The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. A pseudogene has been identified on chromosome 12. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 43 kDa |
| PTM | Acetylated on lysine residues. BLOC1S1 is required for acetylation. |
Images (2) Click the Picture to Zoom In
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ARG65569 anti-NDUFA9 antibody WB image
Western blot: 40 μg of 1) Mouse heart and 2) Mouse kidney lysates stained with ARG65569 anti-NDUFA9 antibody at 1:400 dilution. Exposure time: 10 seconds.
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ARG65569 anti-NDUFA9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG65569 anti-NDUFA9 antibody (left) at 1:30 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
