ARG65568

anti-NDUFA8 antibody

anti-NDUFA8 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFA8
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFA8
Antigen Species Human
Immunogen Synthetic peptide of human NDUFA8
Conjugation Un-conjugated
Alternate Names Complex I-PGIV; NADH-ubiquinone oxidoreductase 19 kDa subunit; CI-19kD; CI-19KD; PGIV; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; CI-PGIV; Complex I-19kD

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P50-200
WB500-2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T cell

Properties

Form Liquid
Purification Purified by antigen-affinity chromatography.
Buffer 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 40% Glycerol
Concentration 2.7 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4702 Human NDUFA8

Swiss-port # P51970 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Gene Symbol NDUFA8
Gene Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa
Background The protein encoded by this gene belongs to the complex I 19 kDA subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]
Highlight Related products:
Anti-Rabbit IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 20 kDa
PTM May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.

Images (3) Click the Picture to Zoom In

  • ARG65568 anti-NDUFA8 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate cancer tissue stained with ARG65568 anti-NDUFA8 antibody (left) at 1:40 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).

  • ARG65568 anti-NDUFA8 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate cancer tissue stained with ARG65568 anti-NDUFA8 antibody (left) at 1:40 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).

  • ARG65568 anti-NDUFA8 antibody WB image

    Western blot: 40 μg of 293T cell lysate stained with ARG65568 anti-NDUFA8 antibody at 1:300 dilution. Exporsure time: 1 minute.