ARG59055
anti-NDUFA7 antibody
anti-NDUFA7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Pig
Overview
| Product Description | Goat Polyclonal antibody recognizes NDUFA7 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Predict Reactivity | Cow |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFA7 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from the internal region of Human NDUFA7. (C-PKLPVGPSHKLSNN, NP_004992.2) |
| Conjugation | Un-conjugated |
| Alternate Names | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; B14.5a; NADH-ubiquinone oxidoreductase subunit B14.5a; CI-B14.5a; Complex I-B14.5a |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 15 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95182 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Swiss-port # Q9Z1P6 Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 |
|---|---|
| Gene Symbol | NDUFA7 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa |
| Background | This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain. [provided by RefSeq, Mar 2011] |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt] |
| Calculated MW | 13 kDa |
Images (3) Click the Picture to Zoom In
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ARG59055 anti-NDUFA7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart stained with ARG59055 anti-NDUFA7 antibody at 5 µg/ml dilution. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
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ARG59055 anti-NDUFA7 antibody WB image
Western blot: 35 µg of Human heart, Mouse heart and Rat heart lysates (in RIPA buffer) stained with ARG59055 anti-NDUFA7 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG59055 anti-NDUFA7 antibody WB image
Western blot: 35 µg of Pig heart lysate (in RIPA buffer) stained with ARG59055 anti-NDUFA7 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
