ARG43936

anti-NDUFA7 antibody

anti-NDUFA7 antibody for ELISA,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFA7
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFA7
Antigen Species Human
Immunogen Human NDUFA7 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
Alternate Names NDUFA7; NADH:Ubiquinone Oxidoreductase Subunit A7; B14.5a; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 7, 14.5kDa; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 7; NADH-Ubiquinone Oxidoreductase Subunit B14.5a; Complex I B14.5a Subunit; CI-B14.5a; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 7 (14.5kD, B14.5a); Complex I-B14.5a

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6 cells
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 4701 Human NDUFA7

GeneID: 66416 Mouse NDUFA7

Swiss-port # O95182 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

Swiss-port # Q9Z1P6 Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

Gene Symbol NDUFA7
Gene Full Name NADH:Ubiquinone Oxidoreductase Subunit A7
Background This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Localization Membrane, Mitochondrion, Mitochondrion inner membrane
Calculated MW 13 kDa
PTM Acetylation, Phosphoprotein

Images (5) Click the Picture to Zoom In

  • ARG43936 anti-NDUFA7 antibody IHC-P image

    Immunohistochemistry: Rat cardiac node metastasis carcinoma stained with ARG43936 anti-NDUFA7 antibody at 2 μg/ml dilution.

  • ARG43936 anti-NDUFA7 antibody WB image

    Western blot: 293T, PC-3 and HepG2 stained with ARG43936 anti-NDUFA7 antibody at 0.5 μg/mL dilution.

  • ARG43936 anti-NDUFA7 antibody FACS image

    Flow Cytometry: SiHa cells stained with ARG43936 anti-NDUFA7 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43936 anti-NDUFA7 antibody WB image

    Western blot: Rat hear and Rat kidney stained with ARG43936 anti-NDUFA7 antibody at 0.5 μg/mL dilution.

  • ARG43936 anti-NDUFA7 antibody WB image

    Western blot: Mouse heart stained with ARG43936 anti-NDUFA7 antibody at 0.5 μg/mL dilution.