ARG65566
anti-NDUFA5 antibody
anti-NDUFA5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Controls and Markers antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFA5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFA5 |
| Antigen Species | Human |
| Immunogen | Fusion protein of human NDUFA5 |
| Conjugation | Un-conjugated |
| Alternate Names | CI-13kD-B; NADH-ubiquinone oxidoreductase 13 kDa-B subunit; UQOR13; Complex I subunit B13; CI-13kB; CI-13KD-B; B13; NUFM; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; Complex I-13kD-B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human hepatocellular carcinoma and fetal brain tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 2.8 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16718 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 |
|---|---|
| Gene Symbol | NDUFA5 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 |
| Background | This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody |
| Calculated MW | 13 kDa |
Images (3) Click the Picture to Zoom In
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ARG65566 anti-NDUFA5 antibody WB image
Western blot: 40 μg of 1) Human hepatocellular carcinoma tissue and 2) Human fetal brain tissue lysates stained with ARG65566 anti-NDUFA5 antibody at 1:400 dilution. Exposure time: 20 seconds.
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ARG65566 anti-NDUFA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG65566 anti-NDUFA5 antibody (left) at 1:60 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: ×200).
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ARG65566 anti-NDUFA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG65566 anti-NDUFA5 antibody (left) at 1:60 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: ×200).
