ARG43935
anti-NDUFA5 antibody
anti-NDUFA5 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFA5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | NDUFA5 |
| Antigen Species | Human |
| Immunogen | Human NDUFA5 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 |
| Alternate Names | NDUFA5; NADH:Ubiquinone Oxidoreductase Subunit A5; NADH-Ubiquinone Oxidoreductase 13 KDa-B Subunit; CI-13KD-B; CI-13kB; UQOR13; NUFM; B13; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5, 13kDa; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 5; Complex I 13kDa Subunit B; Complex I Subunit B13; Ubiquinone Reductase; Type I Dehydrogenase; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5 (13kD, B13); NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5; Complex I-13kD-B; CI-13kD-B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NDUFA5 |
| Gene Full Name | NADH:Ubiquinone Oxidoreductase Subunit A5 |
| Background | This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Cellular Localization | Membrane, Mitochondrion, Mitochondrion inner membrane |
| Calculated MW | 13 kDa |
| PTM | Acetylation |
Images (5) Click the Picture to Zoom In
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ARG43935 anti-NDUFA5 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG43935 anti-NDUFA5 antibody at 5 μg/ml dilution.
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ARG43935 anti-NDUFA5 antibody WB image
Western blot: Rat heart stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.
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ARG43935 anti-NDUFA5 antibody FACS image
Flow Cytometry: U937 cells stained with ARG43935 anti-NDUFA5 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43935 anti-NDUFA5 antibody WB image
Western blot: Hela and K562 stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.
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ARG43935 anti-NDUFA5 antibody WB image
Western blot: Mouse heart stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.
