ARG43935

anti-NDUFA5 antibody

anti-NDUFA5 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFA5
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFA5
Antigen Species Human
Immunogen Human NDUFA5 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
Alternate Names NDUFA5; NADH:Ubiquinone Oxidoreductase Subunit A5; NADH-Ubiquinone Oxidoreductase 13 KDa-B Subunit; CI-13KD-B; CI-13kB; UQOR13; NUFM; B13; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5, 13kDa; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 5; Complex I 13kDa Subunit B; Complex I Subunit B13; Ubiquinone Reductase; Type I Dehydrogenase; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5 (13kD, B13); NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 5; Complex I-13kD-B; CI-13kD-B

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 25488 Rat NDUFA5

GeneID: 4698 Human NDUFA5

GeneID: 68202 Mouse NDUFA5

Gene Symbol NDUFA5
Gene Full Name NADH:Ubiquinone Oxidoreductase Subunit A5
Background This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cellular Localization Membrane, Mitochondrion, Mitochondrion inner membrane
Calculated MW 13 kDa
PTM Acetylation

Images (5) Click the Picture to Zoom In

  • ARG43935 anti-NDUFA5 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43935 anti-NDUFA5 antibody at 5 μg/ml dilution.

  • ARG43935 anti-NDUFA5 antibody WB image

    Western blot: Rat heart stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.

  • ARG43935 anti-NDUFA5 antibody FACS image

    Flow Cytometry: U937 cells stained with ARG43935 anti-NDUFA5 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43935 anti-NDUFA5 antibody WB image

    Western blot: Hela and K562 stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.

  • ARG43935 anti-NDUFA5 antibody WB image

    Western blot: Mouse heart stained with ARG43935 anti-NDUFA5 antibody at 0.5 μg/mL dilution.