ARG65565
anti-NDUFA4 antibody
anti-NDUFA4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFA4 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFA4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of human NDUFA4 |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c oxidase subunit NDUFA4; Complex I-MLRQ; MLRQ; CI-9k; NADH-ubiquinone oxidoreductase MLRQ subunit; CI-MLRQ |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | SKOV3 cell and human fetal liver tissue, human fetal brain tissue and HeLa cell |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 2.3 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00483 Human Cytochrome c oxidase subunit NDUFA4 Swiss-port # Q62425 Mouse Cytochrome c oxidase subunit NDUFA4 |
|---|---|
| Gene Symbol | NDUFA4 |
| Gene Full Name | NDUFA4, mitochondrial complex associated |
| Background | The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Function | Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 9 kDa |
Images (3) Click the Picture to Zoom In
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ARG65565 anti-NDUFA4 antibody WB image
Western blot: 40 μg of 1) SKOV3, 2) Human fetal liver tissue, 3) Human fetal brain tissue, and 4) HeLa lysates stained with ARG65565 anti-NDUFA4 antibody at 1:300 dilution. Exposure time: 20 seconds.
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ARG65565 anti-NDUFA4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG65565 anti-NDUFA4 antibody (left) at 1:40 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
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ARG65565 anti-NDUFA4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG65565 anti-NDUFA4 antibody (left) at 1:40 dilution, or the same antibody preincubated with synthetic peptide (right). (Original magnification: ×200).
