ARG40618

anti-NDUFA4 antibody

anti-NDUFA4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFA4
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFA4
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-81 of Human NDUFA4 (NP_002480.1).
Conjugation Un-conjugated
Alternate Names Cytochrome c oxidase subunit NDUFA4; Complex I-MLRQ; MLRQ; CI-9k; NADH-ubiquinone oxidoreductase MLRQ subunit; CI-MLRQ

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control U-251MG
Observed Size 12 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17992 Mouse NDUFA4

GeneID: 4697 Human NDUFA4

Swiss-port # O00483 Human Cytochrome c oxidase subunit NDUFA4

Swiss-port # Q62425 Mouse Cytochrome c oxidase subunit NDUFA4

Gene Symbol NDUFA4
Gene Full Name NDUFA4, mitochondrial complex associated
Background The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]
Function Cytochrome c oxidase (COX, complex IV) is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. Required for complex IV maintenance. [UniProt]
Cellular Localization Mitochondrion inner membrane; Peripheral membrane protein; Matrix side. [UniProt]
Calculated MW 9 kDa

Images (1) Click the Picture to Zoom In

  • ARG40618 anti-NDUFA4 antibody WB image

    Western blot: 25 µg of U-251MG cell lysate stained with ARG40618 anti-NDUFA4 antibody at 1:1000 dilution.