ARG65563
anti-NDUFA13 antibody
anti-NDUFA13 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NDUFA13 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NDUFA13 |
| Antigen Species | Human |
| Immunogen | Fusion protein of human NDUFA13 |
| Conjugation | Un-conjugated |
| Alternate Names | CI-B16.6; NADH-ubiquinone oxidoreductase B16.6 subunit; CGI-39; Gene associated with retinoic and interferon-induced mortality 19 protein; Cell death regulatory protein GRIM-19; Complex I-B16.6; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; GRIM19; CDA016; B16.6; GRIM-19; Gene associated with retinoic and IFN-induced mortality 19 protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse spleen and skeletal muscle tissue, Human hepatocellular carcinoma, Mouse liver and Human placenta tissue, HeLa and 293T cell tissue. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by antigen-affinity chromatography. |
| Buffer | 1XPBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9ERS2 Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Swiss-port # Q9P0J0 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 |
|---|---|
| Gene Symbol | NDUFA13 |
| Gene Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13 |
| Background | This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes. [UniProt] |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 17 kDa |
Images (3) Click the Picture to Zoom In
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ARG65563 anti-NDUFA13 antibody WB image
Western blot: 40 μg of 1) Mouse spleen, 2) Mouse skeletal muscle, 3) Human hepatocellular carcinoma, 4) Mouse liver, 5) Human placenta, 6) HeLa, and 7) 293T lysates stained with ARG65563 anti-NDUFA13 antibody at 1:350 dilution. Exposure time: 30 seconds.
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ARG65563 anti-NDUFA13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65563 anti-NDUFA13 antibody (left) at 1:40 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: X200).
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ARG65563 anti-NDUFA13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue stained with ARG65563 anti-NDUFA13 antibody (left) at 1:40 dilution, or the same antibody preincubated with fusion protein (right). (Original magnification: X200).
