ARG22547
anti-NCR1 antibody [AKS1] (PE)
anti-NCR1 antibody [AKS1] (PE) for Flow cytometry and Bovine
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [AKS1] recognizes NCR1 This antibody recognizes bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (Sivori et al. 1997). The bovine homologue is expressed on bovine NK cells (Storset et al. 2004) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells (Storset et al. 2004). |
|---|---|
| Tested Reactivity | Bov |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AKS1 |
| Isotype | IgG1 |
| Target Name | NCR1 |
| Antigen Species | Bovine |
| Immunogen | Fusion protein consisting of the extracellular region of bovine CD335. |
| Conjugation | PE |
| Alternate Names | CD antigen CD335; Natural killer cell p46-related protein; Lymphocyte antigen 94 homolog; Natural cytotoxicity triggering receptor 1; hNKp46; NKP46; NKp46; NK cell-activating receptor; LY94; CD335; NK-p46 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q863H2 Bovine Natural cytotoxicity triggering receptor 1 |
|---|---|
| Gene Symbol | NCR1 |
| Gene Full Name | natural cytotoxicity triggering receptor 1 |
| Function | Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. [UniProt] |
| Calculated MW | 34 kDa |
| PTM | N-glycosylated. O-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG22547 anti-NCR1 antibody [AKS1] (PE) FACS image
Flow Cytometry: Figure A. ARG22547 anti-NCR1 antibody [AKS1] (PE) and FITC conjugated Mouse IgG1 isotype control. Figure B. ARG22547 anti-NCR1 antibody [AKS1] (PE) and FITC conjugated Mouse anti Bovine CD2. All experiments performed on red cell lysed Bovine blood gated on mononuclear cells.
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ARG22547 anti-NCR1 antibody [AKS1] (PE) FACS image
Flow Cytometry: Figure A. FITC conjugated Mouse anti Bovine CD2 and PE-conjugated Mouse IgG1 isotype control. Figure B. FITC conjugated Mouse anti Bovine CD2 and ARG22547 anti-NCR1 antibody [AKS1] (PE). All experiments performed on red cell lysed Bovine blood gated on mononuclear cells.
