ARG65814
anti-NCOR2 / SMRT antibody
anti-NCOR2 / SMRT antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit polyclonal antibody recognizes NCOR2 / SMRT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Recognizes endogenous levels of SMRT protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NCOR2 / SMRT |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around the center region of Human NCOR2 / SMRT. |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclear receptor corepressor 2; Silencing mediator of retinoic acid and thyroid hormone receptor; TNRC14; TRAC; N-CoR2; Thyroid-, retinoic-acid-receptor-associated corepressor; CTG repeat protein 26; SMAP270; TRAC1; T3 receptor-associating factor; CTG26; TRAC-1; SMRT; SMRTE-tau; SMRTE |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Sodium citrate buffer (10mM, pH 6.0), Boiling bathing for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | Liquid (pH 7.3), 0.42% Potassium phosphate, 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NCOR2 |
| Gene Full Name | nuclear receptor corepressor 2 |
| Background | This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011] |
| Function | Transcriptional corepressor. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors. Involved in the regulation BCL6-dependent of the germinal center (GC) reactions, mainly through the control of the GC B-cells proliferation and survival. [UniProt] |
| Calculated MW | 274 kDa |
Images (3) Click the Picture to Zoom In
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ARG65814 anti-NCOR2 / SMRT antibody ICC/IF image
Immunocytochemistry: Formalin-fixed HeLa cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 min and blocked with 3% BSA-PBS for 30 min at RT. Cells were stained with ARG65814 anti-NCOR2 / SMRT antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at RT in the dark. DAPI was used to stain the cell nuclei (blue).
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ARG65814 anti-NCOR2 / SMRT antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer tissue section. Antigen retrieval: Sodium citrate buffer (10mM, pH 6.0), Boiling bathing for 20 min. The section was stained with ARG65814 anti-NCOR2 / SMRT antibody at RT and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG65814 anti-NCOR2 / SMRT antibody WB image
Western blot: HeLa and HEK293T whole cell lysates stained with ARG65814 anti-NCOR2 / SMRT antibody.
