ARG57025
anti-NBL1 antibody [38G8]
anti-NBL1 antibody [38G8] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [38G8] recognizes NBL1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 38G8 |
| Isotype | IgG1, kappa |
| Target Name | NBL1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 18-181 of Human NBL1. |
| Conjugation | Un-conjugated |
| Alternate Names | NB; DAN; NO3; DAND1; D1S1733E |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P41271 Human Neuroblastoma suppressor of tumorigenicity 1 |
|---|---|
| Gene Symbol | NBL1 |
| Gene Full Name | neuroblastoma 1, DAN family BMP antagonist |
| Background | This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants have been identified for this gene. Read-through transcripts between this locus and the upstream mitochondrial inner membrane organizing system 1 gene (GeneID 440574) have been observed. [provided by RefSeq, May 2013] |
| Function | Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process. [UniProt] |
| Calculated MW | 19 kDa |
Images (5) Click the Picture to Zoom In
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ARG57025 anti-NBL1 antibody [38G8] ICC/IF image
Immunoflorescense: Jurkat cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57025 anti-NBL1 antibody [38G8] FACS image
Flow Cytometry: Jurkat cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57025 anti-NBL1 antibody [38G8] WB image
Western blot: 30 µg of Jurkat cell lysate stained with ARG57025 anti-NBL1 antibody [38G8] at 1:1000.
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ARG57025 anti-NBL1 antibody [38G8] ICC/IF image
Immunoflorescense: A549 cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57025 anti-NBL1 antibody [38G8] FACS image
Flow Cytometry: A549 cell line stained with ARG57025 anti-NBL1 antibody [38G8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
