ARG42231
anti-NAT8L antibody
anti-NAT8L antibody for Flow cytometry,ICC/IF and Human,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes NAT8L |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | FACS, ICC/IF |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NAT8L |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide around the internal region of Mouse NAT8L. (C-SVDSRFRGKGIAK) (NP_001001985.3) |
| Conjugation | Un-conjugated |
| Alternate Names | NAT8-LIKE; N-acetyltransferase 8-like protein; NACED; Camello-like protein 3; NAA synthetase; EC 2.3.1.17; CML3; N-acetylaspartate synthetase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NAT8L |
| Gene Full Name | N-acetyltransferase 8-like (GCN5-related, putative) |
| Background | This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynthetic enzyme, catalyzing the NAA synthesis from L-aspartate and acetyl-CoA. NAA is a major storage and transport form of acetyl coenzyme A specific to the nervous system. The gene mutation results in primary NAA deficiency (hypoacetylaspartia). [provided by RefSeq, Dec 2010] |
| Function | Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake. [UniProt] |
| Cellular Localization | Cytoplasm. Membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Rough endoplasmic reticulum membrane; Single-pass membrane protein. Note=Its enzymatic activity contribution is quantitatively larger in mitochondrial compartment than in extramitochondrial compartment. [UniProt] |
| Calculated MW | 33 kDa |
Images (2) Click the Picture to Zoom In
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ARG42231 anti-NAT8L antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HEK293 cells, permeabilized with 0.15% Triton. Cells were stained with ARG42231 anti-NAT8L antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized Goat IgG at 10 µg/ml dilution.
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ARG42231 anti-NAT8L antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Kelly cells, permeabilized with 0.5% Triton. Cells were stained with ARG42231 anti-NAT8L antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa Fluor® 488 labelled secondary antibody. IgG control: Unimmunized Goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
