ARG45684
anti-NANS antibody
anti-NANS antibody for ICC/IF,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NANS |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NANS |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human NANS. |
| Conjugation | Un-conjugated |
| Alternate Names | NANS; N-Acetylneuraminate Synthase; SAS; Sialic Acid Synthase; 3-Deoxy-D-Glycero-D-Galacto-Nononate 9-Phosphate Synthase; N-Acetylneuraminic Acid Phosphate Synthase; N-Acetylneuraminate-9-Phosphate Synthase; N-Acetylneuraminic Acid Synthase; Sialic Acid Phosphate Synthase; Epididymis Secretory Protein Li 100; EC 2.5.1.132; EC 2.5.1.57; HEL-S-100; SEMDCG; SEMDG |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 38 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NANS |
| Gene Full Name | N-Acetylneuraminate Synthase |
| Background | This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the condensation of phosphoenolpyruvate (PEP) and N-acetylmannosamine 6-phosphate (ManNAc-6-P) to synthesize N-acetylneuraminate-9-phosphate (Neu5Ac-9-P). [UniProt] |
| Cellular Localization | Cytoplasm; Cytosol. [UniProt] |
| Calculated MW | 40 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG45684 anti-NANS antibody ICC/IF image
Immunofluorescence: Hela stained with ARG45684 anti-NANS antibody at 5 μg/ml dilution.
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ARG45684 anti-NANS antibody WB image
Western blot: MCF-7 stained with ARG45684 anti-NANS antibody at 0.5 μg/ml dilution.
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ARG45684 anti-NANS antibody WB image
Western blot: Rat brain stained with ARG45684 anti-NANS antibody at 0.5 μg/ml dilution.
