ARG42576
anti-NAA60 / NAT15 antibody
anti-NAA60 / NAT15 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NAA60 / NAT15 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NAA60 / NAT15 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-242 of Human NAA60 / NAT15 (NP_079121.1). |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.3.1.48; N-alpha-acetyltransferase 60; Histone acetyltransferase type B protein 4; N-acetyltransferase 15; NatF catalytic subunit; HAT4; EC 2.3.1.88; NAT15 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse brain | ||||||
| Observed Size | ~ 32 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NAA60 |
| Gene Full Name | N(alpha)-acetyltransferase 60, NatF catalytic subunit |
| Background | This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele. [provided by RefSeq, Nov 2015] |
| Function | N-alpha-acetyltransferase that specifically mediates the acetylation of N-terminal residues of the transmembrane proteins, with a strong preference for N-termini facing the cytosol (PubMed:25732826). Displays N-terminal acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met (PubMed:21750686, PubMed:25732826, PubMed:27550639, PubMed:27320834). Required for normal chromosomal segregation during anaphase (PubMed:21750686). May also show histone acetyltransferase activity; such results are however unclear in vivo and would require additional experimental evidences (PubMed:21981917). [UniProt] |
| Cellular Localization | Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Probably forms a intramembrane hairpin-like structure in the membrane. [UniProt] |
| Calculated MW | 27 kDa |
| PTM | Acetylated: autoacetylation is required for optimal acetyltransferase activity. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG42576 anti-NAA60 / NAT15 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver cancer tissue stained with ARG42576 anti-NAA60 / NAT15 antibody at 1:200 dilution.
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ARG42576 anti-NAA60 / NAT15 antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG42576 anti-NAA60 / NAT15 antibody at 1:1000 dilution.
