ARG42554
anti-NAA10 / ARD1A antibody
anti-NAA10 / ARD1A antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NAA10 / ARD1A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NAA10 / ARD1A |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 135-167 of Human NAA10 / ARD1A. |
| Conjugation | Un-conjugated |
| Alternate Names | N-alpha-acetyltransferase 10; ARD1P; N-terminal acetyltransferase complex ARD1 subunit homolog A; DXS707; EC 2.3.1.88; ARD1; NatA catalytic subunit Naa10; EC 2.3.1.-; ARD1A; TE2; MCOPS1; NATD |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa | ||||||
| Observed Size | ~ 30 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NAA10 |
| Gene Full Name | N(alpha)-acetyltransferase 10, NatA catalytic subunit |
| Background | N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| Function | Catalytic subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19826488, PubMed:19420222, PubMed:20145209, PubMed:27708256, PubMed:25489052, PubMed:29754825). Acetylates amino termini that are devoid of initiator methionine (PubMed:19420222). The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation (PubMed:12464182). Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration (PubMed:19826488). Acetylates, and stabilizes TSC2, thereby repressing mTOR activity and suppressing cancer development (PubMed:20145209). Acetylates HSPA1A and HSPA1B at 'Lys-77' which enhances its chaperone activity and leads to preferential binding to co-chaperone HOPX (PubMed:27708256). Acetylates HIST1H4A (PubMed:29754825). Acts as a negative regulator of sister chromatid cohesion during mitosis (PubMed:27422821). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Also present in the free cytosolic and cytoskeleton-bound polysomes. [UniProt] |
| Calculated MW | 26 kDa |
| PTM | Cleaved by caspases during apoptosis. Phosphorylation by IKBKB/IKKB at Ser-209 promotes its proteasome-mediated degradation. Autoacetylated at Lys-136 which stimulates its catalytic activity. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG42554 anti-NAA10 / ARD1A antibody ICC/IF image
Immunofluorescence: 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U2OS cells stained with ARG42554 anti-NAA10 / ARD1A antibody (green) at 1:25 dilution. DAPI (blue) for nuclear staining.
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ARG42554 anti-NAA10 / ARD1A antibody WB image
Western blot: 20 µg of HeLa whole cell lysate stained with ARG42554 anti-NAA10 / ARD1A antibody at 1:2000 dilution.
