ARG44155

anti-N Cadherin antibody [8C11] (PE)

anti-N Cadherin antibody [8C11] (PE) for Flow cytometry and Human,Mouse

EMT Study antibody; Mesenchymal Markers antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [8C11] recognizes N Cadherin
Tested Reactivity Hu, Ms
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone 8C11
Isotype IgG1, kappa
Target Name N Cadherin
Antigen Species Human
Immunogen Human N-Cadherin (extracellular domain).
Conjugation PE
Alternate Names Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS(pH 7.4) and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1000 Human CDH2

GeneID: 12558 Mouse CDH2

Swiss-port # P15116 Mouse Cadherin-2

Swiss-port # P19022 Human Cadherin-2

Gene Symbol CDH2
Gene Full Name cadherin 2, type 1, N-cadherin (neuronal)
Background N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
Function N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein
Cell membrane; sarcolemma
Cell junction
Cell surface
Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt]
Research Area EMT Study antibody; Mesenchymal Markers antibody
Calculated MW ~ 100 kDa (unmodified), 125-140 kDa (modified).
PTM Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG44155 anti-N Cadherin antibody [8C11] (PE) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG44155 anti-N Cadherin antibody [8C11] (PE) at 10 µl / 100 µl of whole blood dilution.