ARG59943
anti-Myosin light chain 3 antibody
anti-Myosin light chain 3 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Myosin light chain 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Myosin light chain 3 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-195 of Human Myosin light chain 3 (NP_000249.1). |
| Conjugation | Un-conjugated |
| Alternate Names | MLC1SB; Ventricular/slow twitch myosin alkali light chain; VLC1; CMH8; Myosin light chain 3; Myosin light chain 1, slow-twitch muscle B/ventricular isoform; MLC1V; CMLC1; Cardiac myosin light chain 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse lung and HepG2 | ||||||
| Observed Size | 23 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MYL3 |
| Gene Full Name | myosin, light chain 3, alkali; ventricular, skeletal, slow |
| Background | MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
| Function | Regulatory light chain of myosin. Does not bind calcium. [UniProt] |
| Calculated MW | 22 kDa |
| PTM | The N-terminus is blocked. N-terminus is methylated by METTL11A/NTM1. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG59943 anti-Myosin light chain 3 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG59943 anti-Myosin light chain 3 antibody.
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ARG59943 anti-Myosin light chain 3 antibody WB image
Western blot: 25 µg of Mouse lung and HepG2 cell lysates stained with ARG59943 anti-Myosin light chain 3 antibody at 1:1000 dilution.
