ARG57027

anti-Myosin Light Chain 4 antibody [4E8]

anti-Myosin Light Chain 4 antibody [4E8] for Western blot and Human

Overview

Product Description

Mouse Monoclonal antibody [4E8] recognizes Myosin Light Chain 4

Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 4E8
Isotype IgG2a, kappa
Target Name Myosin Light Chain 4
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-197 of Human Myosin Light Chain 4.
Conjugation Un-conjugated
Alternate Names Myosin light chain 1, embryonic muscle/atrial isoform; Myosin light chain alkali GT-1 isoform; PRO1957; GT1; AMLC; Myosin light chain 4; ALC1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4635 Human MYL4

Swiss-port # P12829 Human Myosin light chain 4

Gene Symbol MYL4
Gene Full Name myosin, light chain 4, alkali; atrial, embryonic
Background Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Regulatory light chain of myosin. Does not bind calcium. [UniProt]
Calculated MW 22 kDa

Images (2) Click the Picture to Zoom In

  • ARG57027 anti-Myosin Light Chain 4 antibody [4E8] WB image

    Western blot: 40 µg of HeLa cell lysate stained with ARG57027 anti-Myosin Light Chain 4 antibody [4E8] at 1:1000.

  • ARG57027 anti-Myosin Light Chain 4 antibody [4E8] WB image

    Western blot: 40 µg of HepG2, K562, MCF7, Jurkat, 293T and LnCap cell lysates stained with ARG57027 anti-Myosin Light Chain 4 antibody [4E8] at 1:1000 dilution.