ARG65356
anti-Mycobacterium tuberculosis antigen CFP10 (Rv3874) antibody
anti-Mycobacterium tuberculosis antigen CFP10 (Rv3874) antibody for Western blot and Bacteria
Microbiology and Infectious Disease antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Mycobacterium tuberculosis antigen CFP10 (Rv3874) |
|---|---|
| Tested Reactivity | Bacteria |
| Tested Application | WB |
| Specificity | The polyclonal antibody reacts with CFP10 (10 kDa culture filtrate antigen ESXB; LHP), a 12,3 kDa antigen encoded by gene Rv3874 of Mycobacterium tuberculosis. The antibody recognizes CFP10 of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis; it reacts also with recombinant antigen produced in Escherichia coli. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Mycobacterium tuberculosis antigen CFP10 (Rv3874) |
| Immunogen | CFP10 (Rv3874) of Mycobacterium tuberculosis |
| Conjugation | Un-conjugated |
| Alternate Names | cfp10; lhp |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Under reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from rabbit serum by protein-A afinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | esxB |
| Gene Full Name | ESAT-6-like protein EsxB |
| Research Area | Microbiology and Infectious Disease antibody |
| Calculated MW | 11 kDa |
Images (1) Click the Picture to Zoom In
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ARG65356 anti-Mycobacterium tuberculosis antigen CFP10 (Rv3874) antibody WB image
Western blot: Recombinant proteins stained with ARG65356 anti-Mycobacterium tuberculosis antigen CFP10 (Rv3874) antibody.
Lane 1, 3, 5: cell lysates of non-transfected E. coli (negative controls) Lane 2, 4, 6: cell lysate of CFP10-transfected E. coli; dilution (1, 2) 0.5 µg/ml, (3, 4) 0.2 µg/ml, (5, 6) 0.1 µg/ml.
