ARG54213
anti-Myc tag antibody [9E10] (biotin)
anti-Myc tag antibody [9E10] (biotin) for Flow cytometry and Other
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Biotin-conjugated Mouse Monoclonal antibody [9E10] recognizes Myc tag |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FACS |
| Specificity | The antibody 9E10 may be used to detect the cMyc tag. The cmyc gene (8q24 on human chromosome) is the cellular homologue of the vmyc gene originally isolated from an avian myelocytomatosis virus. The cMyc protein is a transcription factor (nuclear localization). cMyc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of cMyc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclindependent kinase, ERK2 and CJun Nterminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of cMyc is tightly regulated but in human cancers cMyc is frequently deregulated. cMyc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 9E10 |
| Isotype | IgG1 |
| Target Name | Myc tag |
| Antigen Species | Human |
| Immunogen | Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of Human Myc |
| Conjugation | Biotin |
| Alternate Names | c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | MYC |
|---|---|
| Gene Full Name | v-myc avian myelocytomatosis viral oncogene homolog |
| Background | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008] |
| Function | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt] |
| Research Area | Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| PTM | Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome. Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity). |
Clone References
The 9-1-1 checkpoint clamp stimulates DNA resection by Dna2-Sgs1 and Exo1.
IP /
SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner.
WB / Human
The diabetes gene Hhex maintains δ-cell differentiation and islet function.
ChIP /
c-Myc-mediated repression of miR-15-16 in hypoxia is induced by increased HIF-2α and promotes tumor angiogenesis and metastasis by upregulating FGF2.
SUMO localizes to the central element of synaptonemal complex and is required for the full synapsis of meiotic chromosomes in budding yeast.
IHC-Fr / Saccharomyces cerevisiae
Binding of TFIIIC to sine elements controls the relocation of activity-dependent neuronal genes to transcription factories.
ICC/IF / Mouse
The role of 8q24 copy number gains and c-MYC expression in amelanotic cutaneous melanoma.
ICC/IF / Human
Regulation of fertility, survival, and cuticle collagen function by the Caenorhabditis elegans eaf-1 and ell-1 genes.
WB / Invertebrates
SUMOylation promotes de novo targeting of HP1α to pericentric heterochromatin.
WB / Mouse
Antibody library screens using detergent-solubilized mammalian cell lysates as antigen sources.
FACS / Human
STRADalpha deficiency results in aberrant mTORC1 signaling during corticogenesis in humans and mice.
IHC-P / Human
Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo.
WB / Rat
The Drosophila protein palmitoylome: characterizing palmitoyl-thioesterases and DHHC palmitoyl-transferases.
ICC/IF / Fruit fly (Drosophila melanogaster)
Sue1p is required for degradation of labile forms of altered cytochromes C in yeast mitochondria.
WB / Yeast
SNAP receptors implicated in vesicle targeting and fusion.
IP / Bovine
A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.
ICC/IF / Yeast
