ARG51538

anti-Myc phospho (Thr58) antibody

anti-Myc phospho (Thr58) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Myc phospho (Thr58)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Myc
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from Human Myc.
Conjugation Un-conjugated
Alternate Names c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17869 Mouse MYC

GeneID: 24577 Rat MYC

GeneID: 4609 Human MYC

Gene Symbol MYC
Gene Full Name v-myc avian myelocytomatosis viral oncogene homolog
Background Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30012 Phospho Myc Antibody Duo (pS62, pS58)
ARG30124 Phospho c-Myc Antibody Duo (Total, pT58)
ARG30126 Phospho c-Myc Antibody Panel (Total, pT58, pS62)
Related products:
Myc antibodies; Myc ELISA Kits; Myc Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 49 kDa
PTM Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).

Images (2) Click the Picture to Zoom In

  • ARG51538 anti-Myc phospho (Thr58) antibody WB image

    Western blot: Extracts from HeLa cells stained with ARG51538 anti-Myc phospho (Thr58) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).

  • ARG51538 anti-Myc phospho (Thr58) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51538 anti-Myc phospho (Thr58) antibody (left) or the same antibody preincubated with blocking peptide (right).