ARG41275

anti-MyD88 antibody

anti-MyD88 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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Overview

Product Description Rabbit Polyclonal antibody recognizes MyD88
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MyD88
Antigen Species Human
Immunogen Recombinant protein corresponding to A44-F264 of Human MyD88.
Conjugation Un-conjugated
Alternate Names MYD88D; Myeloid differentiation primary response protein MyD88

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 35 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17874 Mouse MYD88

GeneID: 301059 Rat MYD88

GeneID: 4615 Human MYD88

Gene Symbol MYD88
Gene Full Name myeloid differentiation primary response 88
Background This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Function Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. [UniProt]
Cellular Localization Cytoplasm. Nucleus. [UniProt]
Calculated MW 33 kDa

Images (5) Click the Picture to Zoom In

  • ARG41275 anti-MyD88 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41275 anti-MyD88 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG41275 anti-MyD88 antibody WB image

    Western blot: 50 µg of samples under reducing conditions. Rat cardiac muscle, HeLa, MCF7, HepG2, Jurkat and Raji whole cell lysates stained with ARG41275 anti-MyD88 antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG41275 anti-MyD88 antibody FACS image

    Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG41275 anti-MyD88 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG41275 anti-MyD88 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse spleen tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41275 anti-MyD88 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG41275 anti-MyD88 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat lung tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41275 anti-MyD88 antibody at 1 µg/ml dilution, overnight at 4°C.

Specific References

Myd88 Signaling Is Involved in the Inflammatory Response in LPS-Induced Mouse Epididymitis and Bone-Marrow-Derived Dendritic Cells

WB / Mouse

Jin-Chuan Liu et al.
Int. J. Mol. Sci.,  (2023)

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