Overview

Product Description	Rabbit Polyclonal antibody recognizes Mucin 5AC
Tested Reactivity	Hu
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	Mucin 5AC
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human Mucin 5AC.
Conjugation	Un-conjugated
Alternate Names	leB; Major airway glycoprotein; Lewis B blood group antigen; Tracheobronchial mucin; mucin; Mucin-5AC; LeB; MUC5; TBM; Gastric mucin; MUC-5AC; Mucin-5 subtype AC, tracheobronchial

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size	220 - 600 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 4586 Human MUC5AC
Gene Symbol	MUC5AC
Gene Full Name	mucin 5AC, oligomeric mucus/gel-forming
Function	Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system. [UniProt]
Cellular Localization	Secreted. [UniProt]
Calculated MW	586 kDa
PTM	C-, O- and N-glycosylated. O-glycosylated on the Thr-/Ser-rich tandem repeats. C-mannosylation in the Cys-rich subdomains may be required for proper folding of these regions and for export from the endoplasmic reticulum during biosynthesis. Proteolytic cleavage in the C-terminal is initiated early in the secretory pathway and does not involve a serine protease. The extent of cleavage is increased in the acidic parts of the secretory pathway. Cleavage generates a reactive group which could link the protein to a primary amide. [UniProt]

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