Overview

Product Description	Rabbit Polyclonal antibody recognizes Mre11
Tested Reactivity	Hu, Ms, Rat
Tested Application	IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	Mre11
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human Mre11.
Conjugation	Un-conjugated
Alternate Names	HNGS1; MRE11B; Meiotic recombination 11 homolog 1; MRE11 homolog 1; MRE11; MRE11 homolog A; Meiotic recombination 11 homolog A; ATLD; Double-strand break repair protein MRE11A

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 17535 Mouse MRE11A GeneID: 4361 Human MRE11A GeneID: 64046 Rat MRE11A Swiss-port # P49959 Human Double-strand break repair protein MRE11A Swiss-port # Q61216 Mouse Double-strand break repair protein MRE11A Swiss-port # Q9JIM0 Rat Double-strand break repair protein MRE11A More
Gene Symbol	MRE11A
Gene Full Name	MRE11 homolog A, double strand break repair nuclease
Background	This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function	Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. [UniProt]
Cellular Localization	Nucleus. Chromosome, telomere. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents. [UniProt]
Calculated MW	81 kDa

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