ARG64655
anti-Monoglyceride Lipase antibody
anti-Monoglyceride Lipase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Monoglyceride Lipase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_009214.1; NP_001003794.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Monoglyceride Lipase |
| Antigen Species | Human |
| Immunogen | C-QDLPHLVNADGQY |
| Conjugation | Un-conjugated |
| Alternate Names | Lysophospholipase-like; Monoacylglycerol lipase; EC 3.1.1.23; HUK5; Lysophospholipase homolog; MAGL; HU-K5; Monoglyceride lipase; MGL |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 33 kDa |
Images (3) Click the Picture to Zoom In
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ARG64655 anti-Monoglyceride Lipase antibody WB image
Western Blot: 1). Mock transfection; 2) MGLL (RC218358) expressing plasmid transfected HEK293 cell lysate standed with ARG64655 anti-Monoglyceride Lipase antibody
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ARG64655 anti-Monoglyceride Lipase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG64655 anti-Monoglyceride Lipase antibody at 5 µg/ml dilution followed by HRP-staining.
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ARG64655 anti-Monoglyceride Lipase antibody WB image
Western blot: 35 µg of Human frontal cortex (A) and Mouse adipose (B) lysates (in RIPA buffer) stained with ARG64655 anti-Monoglyceride Lipase antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
