ARG56117

anti-Mitochondrial antibody [AE-1]

anti-Mitochondrial antibody [AE-1] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [AE-1] recognizes Mitochondrial
Tested Reactivity Hu
Species Does Not React With Ms, Rat
Tested Application ELISA, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone AE-1
Isotype IgG1, kappa
Target Name Mitochondrial
Antigen Species Human
Immunogen A semi-purified mitochondrial preparation.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF0.5 - 1 µg/ml
IHC-P0.5 - 1 µg/ml
WB0.25 - 0.5 µg/ml
Application Note Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Cellular Localization Cytoplasmic (Mitochondria)

Images (2) Click the Picture to Zoom In

  • ARG56117 anti-Mitochondrial antibody [AE-1] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human pancreas stained with ARG56117 anti-Mitochondrial antibody [AE-1].

  • ARG56117 anti-Mitochondrial antibody [AE-1] IHC-P image

    Immunohistochemistry: Formalin-fixed, paraffin-embedded Human tonsil stained with ARG56117 anti-Mitochondrial antibody [AE-1].