ARG56703
anti-Midkine antibody
anti-Midkine antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Midkine |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Midkine |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human Midkine. (VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD) |
| Conjugation | Un-conjugated |
| Alternate Names | ARAP; Neurite outgrowth-promoting protein; Midkine; MK; Neurite outgrowth-promoting factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; NEGF2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MDK |
| Gene Full Name | midkine (neurite growth-promoting factor 2) |
| Background | This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012] |
| Function | Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development (By similarity). [UniProt] |
| Calculated MW | 16 kDa |
Images (1) Click the Picture to Zoom In
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ARG56703 anti-Midkine antibody standard curve image
Sandwich ELISA: ARG56703 anti-Midkine antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56812 anti-Midkine antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
