ARG59014
anti-Methylmalonyl-CoA mutase antibody
anti-Methylmalonyl-CoA mutase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Methylmalonyl-CoA mutase |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Methylmalonyl-CoA mutase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 33-65 of Human Methylmalonyl-CoA mutase (LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE). |
| Conjugation | Un-conjugated |
| Alternate Names | Methylmalonyl-CoA isomerase; MCM; EC 5.4.99.2; Methylmalonyl-CoA mutase, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P16332 Mouse Methylmalonyl-CoA mutase, mitochondrial Swiss-port # P22033 Human Methylmalonyl-CoA mutase, mitochondrial |
|---|---|
| Gene Symbol | MUT |
| Gene Full Name | methylmalonyl CoA mutase |
| Background | This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008] |
| Function | Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species. [UniProt] |
| Cellular Localization | Mitochondrion matrix. [UniProt] |
| Calculated MW | 83 kDa |
Images (6) Click the Picture to Zoom In
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ARG59014 anti-Methylmalonyl-CoA mutase antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG59014 anti-Methylmalonyl-CoA mutase antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59014 anti-Methylmalonyl-CoA mutase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intetsinal cancer tissue stained with ARG59014 anti-Methylmalonyl-CoA mutase antibody at 1 µg/ml dilution.
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ARG59014 anti-Methylmalonyl-CoA mutase antibody WB image
Western blot: Rat kidney and 22RV1 lysates stained with ARG59014 anti-Methylmalonyl-CoA mutase antibody at 0.5 µg/ml dilution.
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ARG59014 anti-Methylmalonyl-CoA mutase antibody FACS image
Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59014 anti-Methylmalonyl-CoA mutase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59014 anti-Methylmalonyl-CoA mutase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG59014 anti-Methylmalonyl-CoA mutase antibody at 1 µg/ml dilution.
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ARG59014 anti-Methylmalonyl-CoA mutase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue stained with ARG59014 anti-Methylmalonyl-CoA mutase antibody at 1 µg/ml dilution.
