ARG22633

anti-Methylglyoxal antibody [9E7]

anti-Methylglyoxal antibody [9E7] for Flow cytometry,ICC/IF,Western blot and Other

Overview

Product Description Mouse Monoclonal antibody [9E7] recognizes Methylglyoxal
Tested Reactivity Other
Tested Application FACS, ICC/IF, WB
Specificity Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal or 4-Hydroxy nonenal.
Host Mouse
Clonality Monoclonal
Clone 9E7
Isotype IgG2a
Target Name Methylglyoxal
Antigen Species Others
Immunogen Synthetic Methylglyoxal modified KLH.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol.
Preservative 0.9% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG22633 anti-Methylglyoxal antibody [9E7] ICC/IF image

    Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22633 anti-Methylglyoxal antibody [9E7] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A,C,E,G) Untreated. (B,D,F,H) Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Primary antibody. (G,H) Composite. .

  • ARG22633 anti-Methylglyoxal antibody [9E7] FACS image

    Flow Cytometry: 90% Methanol fixed Neuroblastoma cells (SH-SY5Y) stained with ARG22633 anti-Methylglyoxal antibody [9E7] at 1:50 for 30 min on ice. Secondary Antibody: Goat anti-Mouse (PE) at 1:100 for 20 min at RT. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours. Dashed line: Non specific IgG control. Solid line: Primary antibodies.

  • ARG22633 anti-Methylglyoxal antibody [9E7] WB image

    Western blot: 0.5 µg of Methylglyoxal-BSA and BSA stained with ARG22633 anti-Methylglyoxal antibody [9E7] at 1:1000 for 2 hours at RT.