ARG65161
anti-Mesothelin antibody
anti-Mesothelin antibody for Western blot and Human
Cancer antibody; Controls and Markers antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Mesothelin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Mesothelin |
| Antigen Species | Human |
| Immunogen | CPSGKKAREIDES |
| Conjugation | Un-conjugated |
| Alternate Names | MPF; Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SMRP |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
| Research Area | Cancer antibody; Controls and Markers antibody |
| Calculated MW | 69 kDa |
| PTM | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. |
Images (1) Click the Picture to Zoom In
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ARG65161 anti-Mesothelin antibody WB image
Western Blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG65161 anti-MSLN antibody at 0.3 µg/ml dilution.
