ARG43712
anti-Mesothelin antibody
anti-Mesothelin antibody for Immunoprecipitation,Western blot and Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Mesothelin |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Mesothelin |
| Antigen Species | Mouse |
| Immunogen | Purified recombinant fragment corresponding to Mouse Mesothelin. |
| Conjugation | Un-conjugated |
| Protein Full Name | Mesothelin |
| Alternate Names | MPF; Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SMRP |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | NIH3T3, Mouse lung | ||||||
| Observed Size | 70 kDa, 40 kDa (cleaved) |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Msln |
| Gene Full Name | mesothelin |
| Background | This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
| Function | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. [UniProt] |
| Cellular Localization | Cell membrane, Golgi apparatus, Membrane, Secreted |
| Calculated MW | 69 kDa |
| PTM | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG43712 anti-Mesothelin antibody WB image
Western blot: NIH3T3 cell lysate stained with ARG43712 anti-Mesothelin antibody.
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ARG43712 anti-Mesothelin antibody WB image
Western blot: Mouse lung tissue lysate stained with ARG43712 anti-Mesothelin antibody.
