ARG64008
anti-Melanoma gp100 antibody
anti-Melanoma gp100 antibody for Western blot and Human
Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Melanoma gp100 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Melanoma gp100 |
| Antigen Species | Human |
| Immunogen | CPIGENSPLLSGQQ |
| Conjugation | Un-conjugated |
| Alternate Names | Premelanosome protein; SILV; ME20; Melanocyte protein Pmel 17; ME20-M; Secreted melanoma-associated ME20 antigen; 95 kDa melanocyte-specific secreted glycoprotein; Silver locus protein homolog; ME20S; D12S53E; SIL; P1; Melanocyte protein PMEL; PMEL17; ME20-S; Melanoma-associated ME20 antigen; gp100; ME20M; P100; SI; P26; Melanocytes lineage-specific antigen GP100 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011] |
| Research Area | Cancer antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 70 kDa |
| PTM | A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides. N-glycosylated. O-glycosylated; contains sialic acid. |
Images (2) Click the Picture to Zoom In
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ARG64008 anti-Melanoma gp100 antibody WB image
Western blot: 35 µg of Human skin lysate (in RIPA buffer) stained with ARG64008 anti-Melanoma gp100 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG64008 anti-Melanoma gp100 antibody WB image
Western blot: 1). Mock transfection; 2) Human SILV (RC200663) expressing plasmid transfected HEK293 cell lysate standed with ARG64008 anti-Melanoma gp100 antibody.
