ARG10762
anti-MeCP2 phospho (Ser421) antibody [2E2]
anti-MeCP2 phospho (Ser421) antibody [2E2] for Dot blot,ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections) and Mouse
Overview
| Product Description | Mouse Monoclonal antibody [2E2] recognizes MeCP2 phospho (Ser421) |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | Dot, ICC/IF, IHC-FoFr |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2E2 |
| Isotype | IgG1 |
| Target Name | MeCP2 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic phosphopeptide around Ser421 of Human MeCP2. |
| Conjugation | Un-conjugated |
| Alternate Names | MRXSL; RS; MeCp2; Methyl-CpG-binding protein 2; MeCp-2 protein; RTT; AUTSX3; RTS; MRX79; PPMX; MRXS13; MRX16 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification. |
| Buffer | PBS and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MECP2 |
| Gene Full Name | methyl CpG binding protein 2 |
| Background | DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009] |
| Function | Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). [UniProt] |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation. |
Images (2) Click the Picture to Zoom In
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ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] ICC/IF image
Immunocytochemistry: Mouse brain section, which was cut at 45 µM on a vibratome after perfusion with 4% parformaldehyde and overnight fixation, was stained with ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] at 1:1000 dilution. Phospho-MeCP2 at S421 was mainly associated with methylated DNA in nucleus of activated neuron cells (red). Blue shows DAPI staining of nuclear DNA. .
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ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] Dot image
Dot blot: 0.5 µg of Phospho-peptide or Non Phospho-peptide stained with ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] on nitrocellulose membrane.
