ARG22511
anti-Mast Cell Tryptase antibody [AA1]
anti-Mast Cell Tryptase antibody [AA1] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat,Cat,Dog,Monkey
Overview
| Product Description | Mouse Monoclonal antibody [AA1] recognizes Mast Cell Tryptase This antibody recognizes human mast cell tryptase, both alpha and beta isoforms. Mouse anti Mast cel tryptase, clone AA1 is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology (Walls et al. 1990).Tryptases are the products of a number of genes and form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction (Zhang and Timmerman 1997). |
|---|---|
| Tested Reactivity | Hu, Rat, Cat, Dog, Mk |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P |
| Specificity | This antibody recognizes human mast cell tryptase, both alpha and beta isoforms. Including protein Tryptase alpha/beta-1 (TPSAB1) and Tryptase beta-2 (TPSB2). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AA1 |
| Isotype | IgG1 |
| Target Name | Mast Cell Tryptase |
| Antigen Species | Human |
| Immunogen | Human mast cell tryptase purified from human lung tissue. |
| Conjugation | Un-conjugated |
| Alternate Names | tryptaseC; tryptaseB; Tryptase beta-2; TPS2; Tryptase II; EC 3.4.21.59; Tryptase-2; TPS1, TPS2, TPSB1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | TPSAB1 + TPSB2 |
|---|---|
| Gene Full Name | Tryptase alpha/beta-1 + tryptase beta 2 (gene/pseudogene) |
| Background | Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008] |
| Function | Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. [UniProt] |
| Calculated MW | 31 kDa |
Images (1) Click the Picture to Zoom In
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ARG22511 anti-Mast Cell Tryptase antibody [AA1] IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil stained with ARG22511 anti-Mast Cell Tryptase antibody [AA1].
