ARG66128

anti-Maspin antibody

anti-Maspin antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Maspin
Tested Reactivity Hu
Tested Application ELISA, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Maspin
Antigen Species Human
Immunogen E. coli derived recombinant Human Maspin.
(MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP)
Conjugation Un-conjugated
Alternate Names PI-5; PI5; Maspin; Serpin B5; maspin; Peptidase inhibitor 5

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66129 as a detection antibody
IHC-P7.8 ng/ml - 15.625 ng/ml
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5268 Human SERPINB5

Swiss-port # P36952 Human Serpin B5

Gene Symbol SERPINB5
Gene Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 5
Function Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. [UniProt]
Calculated MW 42 kDa

Images (6) Click the Picture to Zoom In

  • ARG66128 anti-Maspin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal breast and Human breast lobular carcinoma in situ. The recommended ARG66128 anti-Maspin antibody concentration is 7.8 ng/ml - 15.625 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • ARG66128 anti-Maspin antibody WB image

    Western blot: 250 - 0.24 ng of Human Maspin stained with ARG66128 anti-Maspin antibody, under non-reducing conditions.

  • ARG66128 anti-Maspin antibody standard curve image

    Sandwich ELISA: ARG66128 anti-Maspin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66129 anti-Maspin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66128 anti-Maspin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal breast and Human breast lobular carcinoma in situ. The recommended ARG66128 anti-Maspin antibody concentration is 7.8 ng/ml - 15.625 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • ARG66128 anti-Maspin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal breast and Human breast lobular carcinoma in situ. The recommended ARG66128 anti-Maspin antibody concentration is 7.8 ng/ml - 15.625 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • ARG66128 anti-Maspin antibody WB image

    Western blot: 250 - 0.24 ng of Human Maspin stained with ARG66128 anti-Maspin antibody, under reducing conditions.