ARG42164
anti-MYL12B antibody
anti-MYL12B antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MYL12B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MYL12B |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide between aa. 1-30 of Human MYL12B. |
| Conjugation | Un-conjugated |
| Alternate Names | MLC-B; MLC20; SHUJUN-1; Myosin regulatory light chain MRLC2; Myosin regulatory light chain 12B; MLC-2; MRLC2; Myosin regulatory light chain 20 kDa; Myosin regulatory light chain 2-B, smooth muscle isoform; MLC-2A |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Human placenta | ||||
| Observed Size | ~ 18 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MYL12B |
| Gene Full Name | myosin, light chain 12B, regulatory |
| Background | This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8. [provided by RefSeq, Dec 2014] |
| Function | Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion. [UniProt] |
| Calculated MW | 20 kDa |
| PTM | Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is reduced following epigallocatechin-3-O-gallate treatment. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG42164 anti-MYL12B antibody WB image
Western blot: 35 µg of Human placenta lysate stained with ARG42164 anti-MYL12B antibody.
