anti-MYH11 / SMMHC antibody [SQab20185]

anti-MYH11 / SMMHC antibody [SQab20185] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab20185] recognizes MYH11 / SMMHC
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab20185
Isotype IgG
Target Name MYH11 / SMMHC
Antigen Species Human
Immunogen Synthetic peptide within aa. 1-100 of Human MYH11 / SMMHC.
Conjugation Un-conjugated
Alternate Names Myosin-11; Myosin heavy chain, smooth muscle isoform; AAT4; SMMHC; FAA4; SMHC; Myosin heavy chain 11

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Liomyoma tissue.


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 4629 Human MYH11

Swiss-port # P35749 Human Myosin-11

Gene Symbol MYH11
Gene Full Name myosin, heavy chain 11, smooth muscle
Background The protein encoded by this gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function Muscle contraction. [UniProt]
Cellular Localization Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. [UniProt]
Calculated MW 227 kDa

Images (1) Click the Picture to Zoom In

  • ARG66752 anti-MYH11 / SMMHC antibody [SQab20185] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human liomyoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66752 anti-MYH11 / SMMHC antibody [SQab20185] at 18°C - 25°C for 30 minutes.