ARG43052
anti-MUC13 antibody
anti-MUC13 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MUC13 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MUC13 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to E226-S312 of Human MUC13. |
| Conjugation | Un-conjugated |
| Alternate Names | MUC-13; DRCC1; Mucin-13; Down-regulated in colon cancer 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MUC13 |
| Gene Full Name | mucin 13, cell surface associated |
| Background | Epithelial mucins, such as MUC13, are a family of secreted and cell surface glycoproteins expressed by ductal and glandular epithelial tissues (Williams et al., 2001 [PubMed 11278439]).[supplied by OMIM, Jul 2008] |
| Function | Epithelial and hemopoietic transmembrane mucin that may play a role in cell signaling. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Apical cell membrane. Secreted. Note=Also exists as a soluble form. [UniProt] |
| Calculated MW | 55 kDa |
| PTM | Cleaved into two subunits, alpha and beta, probably between the first EGF domain and the SEA domain. Beta subunit contains the cytoplasmic tail and alpha subunit the extracellular tail. The homooligomerization into dimers is dependent on intrachain disulfide bonds. Highly N-glycosylated. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human appendicitis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43052 anti-MUC13 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Human placenta, Caco-2, PC-3, U2OS, U-87MG, HepG2 and HeLa whole cell lysates stained with ARG43052 anti-MUC13 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG43052 anti-MUC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43052 anti-MUC13 antibody at 1 µg/ml dilution, overnight at 4°C.
