ARG64519
anti-MTHFS antibody
anti-MTHFS antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes MTHFS |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MTHFS |
| Antigen Species | Human |
| Immunogen | KRCLQHQEVKPYT |
| Conjugation | Un-conjugated |
| Alternate Names | Methenyl-THF synthetase; EC 6.3.3.2; 5-formyltetrahydrofolate cyclo-ligase; HsT19268; MTHFS; 5,10-methenyl-tetrahydrofolate synthetase |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P49914 Human 5-formyltetrahydrofolate cyclo-ligase |
|---|---|
| Background | The protein encoded by this gene is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, a precursor of reduced folates involved in 1-carbon metabolism. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 23 kDa |
Images (2) Click the Picture to Zoom In
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ARG64519 anti-MTHFS antibody WB image
Western Blot: Human Liver lysate (35 µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. stained with ARG64519 anti-MTHFS antibody at 0.5 µg/ml dilution.
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ARG64519 anti-MTHFS antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64519 anti-MTHFS antibody at 5 µg/ml dilution followed by AP-staining.
