ARG42226
anti-MTHFD1L antibody
anti-MTHFD1L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Goat Polyclonal antibody recognizes MTHFD1L |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MTHFD1L |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human MTHFD1L. (C-DTNDRFLRKITIGQ) (NP_056255.2) |
| Conjugation | Un-conjugated |
| Alternate Names | MTC1THFS; dJ292B18.2; Formyltetrahydrofolate synthetase; EC 6.3.4.3; Monofunctional C1-tetrahydrofolate synthase, mitochondrial; FTHFSDC1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 110 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3V3R1 Mouse Monofunctional C1-tetrahydrofolate synthase, mitochondrial Swiss-port # Q6UB35 Human Monofunctional C1-tetrahydrofolate synthase, mitochondrial |
|---|---|
| Gene Symbol | MTHFD1L |
| Gene Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like |
| Background | The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2011] |
| Function | May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2. [UniProt] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 106 kDa |
Images (3) Click the Picture to Zoom In
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ARG42226 anti-MTHFD1L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG42226 anti-MTHFD1L antibody at 5 µg/ml dilution followed by AP-staining.
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ARG42226 anti-MTHFD1L antibody WB image
Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG42226 anti-MTHFD1L antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG42226 anti-MTHFD1L antibody WB image
Western blot: 35 µg of NIH/3T3, Mouse brain and Rat brain lysates (in RIPA buffer) stained with ARG42226 anti-MTHFD1L antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
