ARG44426

anti-MTF1 antibody

anti-MTF1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MTF1
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MTF1
Antigen Species Human
Immunogen Human MTF1 recombinant protein (aa. sequence: A28-D634).
Conjugation Un-conjugated
Alternate Names MTF1; Metal Regulatory Transcription Factor 1; MRE-Binding Transcription Factor; Transcription Factor MTF-1; Metal-Responsive Transcription Factor 1; MRE-Binding Transcription Factor-1; Zinc Regulatory Factor; MTF-1; ZRF

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.1-0.25 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17764 Mouse MTF1

GeneID: 4520 Human MTF1

Swiss-port # Q07243 Mouse Metal regulatory transcription factor 1

Swiss-port # Q14872 Human Metal regulatory transcription factor 1

Gene Symbol MTF1
Gene Full Name Metal Regulatory Transcription Factor 1
Background This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE).
Function Zinc-dependent transcriptional regulator of cellular adaption to conditions of exposure to heavy metals.
Cellular Localization Cytoplasm, Nucleus
Calculated MW 81 kDa
PTM Acetylation, Phosphoprotein

Images (6) Click the Picture to Zoom In

  • ARG44426 anti-MTF1 antibody IHC-P image

    Immunohistochemistry: Human breast cancer stained with ARG44426 anti-MTF1 antibody at 2 μg/mL dilution.

  • ARG44426 anti-MTF1 antibody ICC/IF image

    Immunofluorescence: U87 stained with ARG44426 anti-MTF1 antibody at 5 μg/mL dilution.

  • ARG44426 anti-MTF1 antibody WB image

    Western blot: Jurkat and 293T stained with ARG44426 anti-MTF1 antibody at 0.5 μg/mL dilution.

  • ARG44426 anti-MTF1 antibody FACS image

    Flow Cytometry: 293T stained with ARG44426 anti-MTF1 antibody at 1 μg/10^6 cells dilution.

  • ARG44426 anti-MTF1 antibody WB image

    Western blot: Rat liverand Rat brain stained with ARG44426 anti-MTF1 antibody at 0.5 μg/mL dilution.

  • ARG44426 anti-MTF1 antibody WB image

    Western blot: Mouse liver and Mouse brain stained with ARG44426 anti-MTF1 antibody at 0.5 μg/mL dilution.