Overview

Product Description	Rabbit Polyclonal antibody recognizes MTDH / AEG1
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	MTDH / AEG1
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human MTDH / AEG1.
Conjugation	Un-conjugated
Alternate Names	3D3/LYRIC; AEG-1; Metastasis adhesion protein; 3D3; LYRIC/3D3; AEG1; Protein LYRIC; Lysine-rich CEACAM1 co-isolated protein; LYRIC; Metadherin; Astrocyte elevated gene-1 protein

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:1000 - 1:5000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 75 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 170910 Rat MTDH GeneID: 67154 Mouse MTDH GeneID: 92140 Human MTDH Swiss-port # Q80WJ7 Mouse Protein LYRIC Swiss-port # Q86UE4 Human Protein LYRIC Swiss-port # Q9Z1W6 Rat Protein LYRIC More
Gene Symbol	MTDH
Gene Full Name	metadherin
Function	Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. [UniProt]
Cellular Localization	Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Cell junction, tight junction. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. [UniProt]
Calculated MW	64 kDa

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