ARG58301
anti-MTCO1 antibody
anti-MTCO1 antibody for Immunocytochemistry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MTCO1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MTCO1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence at the C-terminus of Human MTCO1(501-514aa PYHTFEEPVYMKS). |
| Conjugation | Un-conjugated |
| Alternate Names | Cytochrome c oxidase subunit 1; EC 1.9.3.1; COX1; Cytochrome c oxidase polypeptide I; MTCO1; COI |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MT-CO1 |
| Gene Full Name | mitochondrially encoded cytochrome c oxidase I |
| Function | Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane; Multi-pass membrane protein. [UniProt] |
| Calculated MW | 57 kDa |
Images (4) Click the Picture to Zoom In
-
ARG58301 anti-MTCO1 antibody ICC image
Immunocytochemistry: C6 cells stained with ARG58301 anti-MTCO1 antibody.
-
ARG58301 anti-MTCO1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer stained with ARG58301 anti-MTCO1 antibody.
-
ARG58301 anti-MTCO1 antibody WB image
Western blot: SMMC-7721, MCF-7, Raji and SW620 cell lysates stained with ARG58301 anti-MTCO1 antibody.
-
ARG58301 anti-MTCO1 antibody WB image
Western blot: The samples were loaded under reducing conditions. 20 µg of HeLa mitochondria, 20 µg of HeLa, 50 µg of Caco-2, 50 µg of Rat heart and 50 µg of Mouse heart lysates stained with ARG58301 anti-MTCO1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
