ARG41658

anti-MST1R / RON antibody

Datasheet

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100 μl

389.00

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Datasheet

Overview

Product Description	Rabbit Polyclonal antibody recognizes MST1R / RON
Tested Reactivity	Hu
Tested Application	FACS, ICC/IF, IHC-P, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	MST1R / RON
Antigen Species	Human
Immunogen	Synthetic peptide of Human MST1R / RON.
Conjugation	Un-conjugated
Alternate Names	CD136; CDw136; EC 2.7.10.1; RON; PTK8; Protein-tyrosine kinase 8; CD antigen CD136; p185-Ron; Macrophage-stimulating protein receptor; MSP receptor

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1:30
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200
IP	1:30
WB	1:1000 - 1:2000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

SK-BR-3

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 4486 Human MST1R Swiss-port # Q04912 Human Macrophage-stimulating protein receptor
Gene Symbol	MST1R
Gene Full Name	macrophage stimulating 1 receptor
Background	This gene encodes a cell surface receptor for macrophage-stimulating protein (MSP) with tyrosine kinase activity. The mature form of this protein is a heterodimer of disulfide-linked alpha and beta subunits, generated by proteolytic cleavage of a single-chain precursor. The beta subunit undergoes tyrosine phosphorylation upon stimulation by MSP. This protein is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung, and together with MSP, thought to be involved in host defense. Alternatively spliced transcript variants encoding different isoforms with different structural and biochemical properties have been described (PMID:8816464). [provided by RefSeq, Oct 2011]
Function	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand. [UniProt]
Cellular Localization	Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW	152 kDa
PTM	Proteolytic processing yields the two subunits. Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site. Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation. [UniProt]