ARG40105
anti-MRPS35 antibody
anti-MRPS35 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MRPS35 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MRPS35 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-323 of Human MRPS35 (NP_068593.2). |
| Conjugation | Un-conjugated |
| Alternate Names | HDCMD11P; S28mt; MRPS28; MRP-S35; MDS023; 28S ribosomal protein S35, mitochondrial; S35mt; 28S ribosomal protein S28, mitochondrial; MRP-S28 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse kidney and HT-29 | ||||||||
| Observed Size | ~ 38 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P82673 Human 28S ribosomal protein S35, mitochondrial Swiss-port # Q8BJZ4 Mouse 28S ribosomal protein S35, mitochondrial |
|---|---|
| Gene Symbol | MRPS35 |
| Gene Full Name | mitochondrial ribosomal protein S35 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq, Jul 2010] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 37 kDa |
Images (1) Click the Picture to Zoom In
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ARG40105 anti-MRPS35 antibody WB image
Western blot: 25 µg of Mouse kidney and HT-29 cell lysates stained with ARG40105 anti-MRPS35 antibody at 1:1000 dilution.
