ARG42904
anti-MRPS31 antibody
anti-MRPS31 antibody for ICC/IF,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MRPS31 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MRPS31 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 66-395 of Human MRPS31 (NP_005821.2). |
| Conjugation | Un-conjugated |
| Alternate Names | MRP-S31; S31mt; IMOGN38; 28S ribosomal protein S31, mitochondrial; Imogen 38 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Jurkat | ||||||
| Observed Size | ~ 38 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # B0BN56 Rat 28S ribosomal protein S31, mitochondrial Swiss-port # Q92665 Human 28S ribosomal protein S31, mitochondrial |
|---|---|
| Gene Symbol | MRPS31 |
| Gene Full Name | mitochondrial ribosomal protein S31 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that has also been associated with type 1 diabetes; however, its relationship to the etiology of this disease remains to be clarified. Pseudogenes corresponding to this gene have been found on chromosomes 3 and 13. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 45 kDa |
Images (2) Click the Picture to Zoom In
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ARG42904 anti-MRPS31 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG42904 anti-MRPS31 antibody at 1:200 dilution.
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ARG42904 anti-MRPS31 antibody WB image
Western blot: 25 µg of Jurkat cell lysate stained with ARG42904 anti-MRPS31 antibody at 1:3000 dilution.
